Western blot. All lanes: ERO1B antibody at 3.2µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: U251 whole cell lysate. Lane 3: Mouse liver tissue. Lane 4: U87 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 54, 17 kDa. Observed band size: 54 kDa