Western blot. All lanes: IL22RA1 antibody at 10ug/ml. Lane 1: Mouse spleen tissue. Lane 2: HepG2 whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 63 kDa. Observed band size: 63 kDa.