Western blot. All lanes: RPGR antibody at 6µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 114, 91, 71, 84, 53, 128 kDa. Observed band size: 114 kDa