Western Blot. Positive WB detected in: PC3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate. All lanes: TNFRSF25 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46, 24, 28, 20, 15, 14, 26, 41, 42, 30 kDa. Observed band size: 46 kDa.