Western Blot. Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, THP-1 whole cell lysate. All lanes: MICAL1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 118, 109, 38, 120 kDa. Observed band size: 120 kDa.