Western blot. All lanes: YME1L1 antibody at 3.8µg/ml. Lane 1: A549 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: HepG2 whole cell lysate. Lane 6: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 87, 80, 76 kDa. Observed band size: 87 kDa