Western blot. All lanes: Target of rapamycin complex 2 subunit MAPKAP1 antibody at 2ug/ml. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 60, 55, 54, 38, 37, 42 kDa. Observed band size: 60 kDa.