Western Blot. Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, Rat brain tissue, Rat heart tissue. All lanes: TRIM4 antibody at 3.4ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 55, 35 kDa. Observed band size: 55, 62, 35, 90 kDa.