Western blot. All lanes: RAB18 antibody at 0.88µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: PC-3 whole cell lysate. Lane 3: A375 whole cell lysate. Lane 4: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 23, 27, 16 kDa. Observed band size: 23 kDa