Western blot. All lanes: HDHD3 antibody at 1ug/ml. Lane 1: Mouse small intestine tissue. Lane 2: HT29 whole cell lysate. Lane 3: COLO205 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 28 kDa. Observed band size: 28 kDa.