Western blot. All lanes: COPS3 antibody at 3.41µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: 293T whole cell lysate. Lane 4: HT29 whole cell lysate. Lane 5: Mouse heart tissue. Lane 6: Mouse brain tissue. Lane 7: Mouse ovarian tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 48, 46 kDa. Observed band size: 48 kDa