Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate. All lanes: HDAC9 antibody at 1.54μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 112, 102, 66, 98, 118, 113, 61, 63, 58 KDa. Observed band size: 160 KDa.