Western Blot. Positive WB detected in A549 whole cell lysate(treated with Calyculin A or not). All lanes Phospho-NFKBIA antibody at 1.07μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 KDa. Observed band size: 39 KDa.