Western Blot. Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF). All lanes Phospho-RAF1 antibody at 1.18μg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 73 KDa. Observed band size: 73 KDa.