Western Blot. Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate 293 whole cell lysate, A549 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate. All lanes: Id1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 17, 16 kDa. Observed band size: 26 kDa.