Western Blot. Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, Ntera-2 whole cell lysate, U251 whole cell lysate. All lanes: PSME1 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 27 kDa. Observed band size: 28-30, 55-72 kDa.