Western Blot. Positive WB detected in: 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, Mouse Brain whole cell lysate. All lanes: PKC antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 82, 75, 68 kDa. Observed band size: 82, 55 kDa.