Product & ReviewsAntibodies

Cy3-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG, Fc Fragment Specific (min X Hu Sr Prot)

Product Details

Cat. No.
111-166-046
Type
Secondary Antibody
Clonality
Polyclonal
Host
Goat
Buy Now
Jackson ImmunoResearch Laboratories Inc.

Receive your quote directly from the manufacturer.

Description

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of rabbit IgG heavy chain but not with the Fab portion of rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but it may cross-react with immunoglobulins from other species.

Biological Information

  • Clonality: Polyclonal
  • Host: Goat
  • Reactivity: Rabbit (Lapine)

Handling

  • Quantity: 1 mg
  • Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended us
  • Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
  • Specificity: 5

Applications

    Average Rating 5.0

    |1Scientists have reviewed this product

    Specificity rating
    Sensitivity rating
    Quality rating
    Write your own review

    reliable secondary antibody

     

    Average Rating 5.0

    Application Area:

    Immunofluorescence

    I always use the secondary antibodies produced by Jackson ImmunoResearch Laboratories. I have learned this from my former lab. Their products never let us down.

    Review Date: 24 Oct 2022 | Jackson ImmunoResearch Laboratories Inc.

    Product Overview

    Links