CyFlow™ Beta-catenin Purified

β-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound α, β and γ catenin. β-catenin links this complex through α-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumor cells. β-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 β. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of β-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Hamster, Human, Mouse
• Antigen: β-catenin
• Clone: EM-22
• Conjugate: Purified
• Isotype: IgG1

• Quantity: 0.1 mg
• Storage: Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
• Buffer: The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.

• Flow Cytometry (FC)
• Immunocytochemistry (ICC)
• Immunoprecipitation (IP)
• Western Blotting (WB)