CyFlow™ CD28 Low Endotoxin

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express α/β and γ/δ TCR. The level of CD28 on mature CD4+ and CD8+ α/β TCR+ thymocytes is two- to fourfold lower than on the immature cells.

• Clonality: Monoclonal
• Host: Hamster
• Reactivity: Mouse
• Antigen: CD28
• Clone: 37.51
• Conjugate: Low Endotoxin
• Isotype: IgG

• Quantity: 0.1 mg
• Storage: Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
• Buffer: The reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

• Flow Cytometry (FC)
• Functional Studies (Func)
• Immunocytochemistry (ICC)
• Immunohistochemistry (Formalin-Fixed Sections) (IHC (F))
• Immunoprecipitation (IP)