CyFlow™ CD43 Pacific Blue™

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin, CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human
• Antigen: CD43
• Clone: MEM-59
• Conjugate: Pacific Blue™
• Isotype: IgG1

• Quantity: 100 tests
• Storage: Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
• Buffer: The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.

• Flow Cytometry (FC)
• Functional Studies (Func)
• Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
• Immunoprecipitation (IP)
• Western Blotting (WB)