CyFlow™ CD47 Pacific Blue™

CD47 (IAP; integrin-associated protein) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (SIRP α; signal regulatory protein α), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human, Non-Human Primate, Pig (Swine)
• Antigen: CD47
• Clone: MEM-122
• Conjugate: Pacific Blue™
• Isotype: IgM

• Quantity: 100 tests
• Storage: Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
• Buffer: The reagent is provided in stabilizing Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.1% (w/v) sodium azide.

• Flow Cytometry (FC)
• Immunohistochemistry (Formalin-Fixed Sections) (IHC (F))
• Immunohistochemistry (Frozen Sections) (IHC (Fr))
• Western Blotting (WB)