Product & ReviewsAntibodies

CyFlow™ CD47 Purified

Product Details

Cat. No.
AV372758
Type
Primary Antibody
Clonality
Monoclonal
Host
Mouse
Buy Now
Sysmex Europe SE

Receive your quote directly from the manufacturer.

Description

CD47 (IAP; integrin-associated protein) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (SIRP α; signal regulatory protein α), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

Biological Information

  • Clonality: Monoclonal
  • Host: Mouse
  • Reactivity: Human, Non-Human Primate, Pig (Swine)
  • Antigen: CD47
  • Clone: MEM-122
  • Conjugate: Purified
  • Isotype: IgM

Handling

  • Quantity: 0.1 mg
  • Storage: Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
  • Buffer: The reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.1% (w/v) sodium azide.

Applications

  • Flow Cytometry (FC)
  • Immunohistochemistry (Formalin-Fixed Sections) (IHC (F))
  • Immunohistochemistry (Frozen Sections) (IHC (Fr))
  • Western Blotting (WB)
Specificity rating
Sensitivity rating
Quality rating
Write your own review

Product Overview

Links