CyFlow™ CD50 Low Endotoxin

CD50 ( ICAM-3; intracellular adhesion molecule 3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (DC-SIGN; dendritic cell-specific ICAM-3-grabbing nonintegrin), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human
• Antigen: CD50
• Clone: MEM-04
• Conjugate: Low Endotoxin
• Isotype: IgG1

• Quantity: 0.1 mg
• Storage: Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
• Buffer: The reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

• Flow Cytometry (FC)
• Functional Studies (Func)
• Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))