CyFlow™ CD86 Low Endotoxin

CD86 (B7-2) and CD80 (B7-1) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.

• Clonality: Monoclonal
• Host: Rat
• Reactivity: Mouse
• Antigen: CD86
• Clone: GL-1
• Conjugate: Low Endotoxin
• Isotype: IgG2a

• Quantity: 0.1 mg
• Storage: Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
• Buffer: The reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

• Flow Cytometry (FC)
• Functional Studies (Func)
• Immunocytochemistry (ICC)
• Immunohistochemistry (Formalin-Fixed Sections) (IHC (F))
• Immunoprecipitation (IP)