Direct-Blot™ HRP anti-β-actin
Product Details
- Cat. No.
- 643807
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
Direct-Blot™ HRP anti-β-actin; Clone: 2F1-1; Reactivity: Human, Mouse; Apps: WB; Size: 100 μl (20 Western blots)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Human, Mouse
- Clone: 2F1-1
- Conjugate: DB_HRP
- Isotype: Mouse IgG2b, κ
- Gene: 11461
Handling
- Quantity: 100 microl (20 Western blots)
- Storage: -20°C
- Specificity: 5
Applications
- Western Blotting (WB)
Performs well with clear results
Western Blot
We used this antibody as a faster method to see our protein loading concentrations. Instead of incubating overnight, we incubate for 1 hr at room temp. The sensitivity and specificity were comparable to our other actin controls (overnight incubation with secondary HRP antibody). We used a 1:80000 dilution with about 20 micrograms of protein loaded.
Review Date: 24 Sept 2018 | BioLegend
Performs well with no any viriability
Western blot
This antibody is easy to handle, it has decent sensitivity, we use them as a control to test in vivo protein degradation, the result is clean and clear.
Review Date: 29 Nov 2017 | BioLegend
