DNER antibody
Product Details
- Cat. No.
- orb215597
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
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Description
Rabbit polyclonal antibody to DNER
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Human, Mouse, Rat
Handling
- Specificity: 2
Applications
- Western Blotting (WB)
Sensitive, but not very specific (could be batch-dependent).
Western blot
The antibody was very sensitive, but not specific. Three different bands appeared on sizes: the strongest band (60 kDa) and two bands of equal intensity (70, 80 kDa). Also, it seems that this batch is very sensitive to thawing and freezing.
Review Date: 27 Sept 2020 | Biorbyt
Performs very effectively, but with partial accuracy in used cell systems.
Western blot
I tested the antibody on human ovarian cancer cell extracts and A549 cell line. Samples were collected, sonicated, mixed with loading buffer, and resolved on a vertical electrophoresis system using denaturating conditions. I incubated the nitrocellulose membrane using 1:1000 antibody diluted in 5% non-fat milk with TBS-T for 2 h on rt, washed in TBS-T and incubated with adequate secondary antibody. Visualization was done using ECL. Signals were strong, but there was three of them, between the sizes of 80-50 kDa. The weakest signal was around 78 kDa, which is the predicted size for DNER protein. Changes in protocol did not alter the result.
Review Date: 1 Jul 2020 | Biorbyt
