Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key emzymes in glycolysis. GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). GAPDH is also involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH is constitutively expressed at high level in almost all tissue and cell lines making an ideal positive control marker in immunotechniques. This antibody works best for ELISA and immunohistochemistry.
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Mouse, Rat
- Antigen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of mouse GAPDH.
- Isotype: Rabbit Ig
- Gene: P16858
- Quantity: 0.1 mg
- Storage: Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.
- ELISA (ELISA)
- Immunohistochemistry (IHC)
- Western Blotting (WB)