GOAT ANTI AIF1 (C-TERMINAL)

Goat anti AIF1 antibody recognizes human allograft inflammatory factor 1 (AIF1), otherwise known as Ionized calcium-binding adapter molecule 1 (IBA1). AIF1 is a 147 amino acid ~17kDa actin-binding protein involved in macrophage activation, vascular smooth muscle cell proliferation and activation and T-lymphocyte proliferation (UniProt P55008). Goat anti AIF1 antibody recognizes epitopes at the C-terminal region of AIF1 and is expected to recognize AIF1 isoform 1 and isoform 3 formed by alternative splicing.AIF1 was first identified in rat cardiac allografts with chronic rejection (Utans et al. 1995), is expressed by activated monocytes and macrophages (Orsmark et al. 2007) and plays a significant role in vascular inflammation (Chen et al. 2004) Goat anti AIF1 has been used successfully for the identification of AIF1 expressing cells in an inflammatory model in formalin fixed mouse brain following heat mediated antigen retrieval with sodium citrate buffer by immunohistochemistry (Wang et al. 2014).

• Clonality: Polyclonal
• Host: Goat
• Reactivity: Human, Mouse, Pig (Swine), Rat
• Isotype: Polyclonal IgG

• Quantity: 0.1 mg
• Storage: +4ºC, -20ºC if preferred
• Buffer: TRIS buffered saline, 0.02% Sodium Azide, 0.5% BSA

• ELISA (ELISA)
• Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
• Western Blotting (WB)