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Product & ReviewsAntibodies

Goat Anti-Human IgG, Fc Fragment Specific

Jackson ImmunoResearch Laboratories Inc.
5.0/5.0
|1 Review|Write your own Review

Product Details

Cat. No.
109-001-008
Type
Secondary Antibody
Clonality
Polyclonal
Host
Goat
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Jackson ImmunoResearch Laboratories Inc.

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Product Overview

Goat Anti-Human IgG, Fc Fragment Specific

Jackson ImmunoResearch Laboratories Inc.
5.0/5.0
|0 Reviews
Buy Now

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Jackson ImmunoResearch Laboratories Inc.

JIR anti-Fc robust and stable capture!

Rupesh Nanjunda  Janssen


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  • Summary
  • Reviews

Description

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.Based on immunoelectrophoresis, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human immunoglobulins. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Biological Information

  • Clonality: Polyclonal
  • Host: Goat
  • Reactivity: Human

Handling

  • Quantity: 2 ml
  • Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
  • Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
  • Specificity: 5

Applications

    Average Rating 5.0

    |1Scientists have reviewed this product

    Specificity rating
    Sensitivity rating
    Quality rating
    Write your own review

    JIR anti-Fc robust and stable capture!

     

    Average Rating 5.0

    Application Area:

    Capture reagent

    The anti-human polyclonal capture reagents are some of the most robust reagents I have in my biophysical and biochemical studies so far. The capture is very stable for a long time (hours) in an experiment, the anti-human coated chips/plates can be re-used multiple times (sometimes for weeks to a month) without losing antibody capturing capacity and chip surface integrity. Overall, the anti-Fc reagents regardless of the host or capturing species provides a robust capturing ability with minimum cross-reactivity to other species.

    Review Date: 27 Jan 2021 | Jackson ImmunoResearch Laboratories Inc.

    Rupesh Nanjunda

    • Organization: Janssen
    • Specificity rating
    • Sensitivity rating
    • Quality rating