HIF-1 alpha Antibody (H1alpha67)
Product Details
- Cat. No.
- NB100-123
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7425c2ef2c3e59c2f444a40f1e36809040da7956-265x400.jpg?w=1080&q=75&fit=clip&auto=format)
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7425c2ef2c3e59c2f444a40f1e36809040da7956-265x400.jpg?w=256&q=75&fit=clip&auto=format)
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Description
HIF-1 alpha Antibody (H1alpha67)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Avian, Human, Mouse, Pig (Swine)
- Antigen: HIF-1 alpha
- Isotype: IgG2b
- Source: Protein A purified
Handling
- Quantity: 0.1 ml
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: PBS with 1% BSA
Applications
- Chromatin Immunoprecipitation (ChIP)
- ELISA (ELISA)
- Flow Cytometry (FC)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Immunoprecipitation (IP)
- Western Blotting (WB)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Analysis using the HRP conjugate of NB100-123. On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution (NB100-123). The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/7425c2ef2c3e59c2f444a40f1e36809040da7956-265x400.jpg?w=3840&q=75&fit=clip&auto=format)