HIF-1 alpha [Hydroxy Pro564] Antibody
Product Details
- Cat. No.
- NB110-74679
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - HIF-1alpha HYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4414554a7b2ef1952c8d5317bb170de8bb9b57cd-371x400.jpg?w=1080&q=75&fit=clip&auto=format)
Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - HIF-1alpha HYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - HIF-1alpha HYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4414554a7b2ef1952c8d5317bb170de8bb9b57cd-371x400.jpg?w=256&q=75&fit=clip&auto=format)
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Description
HIF-1 alpha [Hydroxy Pro564] Antibody
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Bovine, Dog (Canine), Human, Mouse
- Antigen: HIF-1 alpha
- Modifications: Hydroxy Pro564
- Source: Whole antisera
Handling
- Quantity: 0.1 ml
- Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
- Buffer: 0.02 M Potassium Phosphate with 0.15 M Sodium Chloride pH 7.2 (Concentration 85 mg/mL by Refractometry)
Applications
- ELISA (ELISA)
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Western Blotting (WB)
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - HIF-1alpha HYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4414554a7b2ef1952c8d5317bb170de8bb9b57cd-371x400.jpg?w=3840&q=75&fit=clip&auto=format)