Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone H2B is ubiquitylated at lysine 120 in vertebrates. H2B ubiquityl Lys120 is found preferentially associated with the transcribed regions of highly expressed genes. It is possible that H2B ubiquitylation is associated with transcriptional elongation.
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Not Species Specific, Human
- Clone: 56
- Isotype: IgG2a
- Quantity: 10 ug
- Storage: Antibodies in solution can be stored at -20C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting (WB)