Histone H3K4me2 rabbit antibody (pAb)
Product Details
- Cat. No.
- 39141
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
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Description
Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Methylation of histone H3 at lysine 4 is linked to transcriptional activation through the recruitment of a number of downstream effector proteins (e.g. CHD1, ING2, Isw1, NURF301, WDR5). Lysine 4 methylation is dependant upon the monoubiquitylation of the C-terminus of histone H2A.
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Not Species Specific, Human, Mouse
- Isotype: Serum
Handling
- Quantity: 200 ul
- Storage: Antibodies in solution can be stored at -20C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Specificity: 5
Applications
- Chromatin Immunoprecipitation (ChIP)
- Dot Blot (DB)
- Western Blotting (WB)
Signals for this antibody are clean and reproducible and match the distribution expectations for this modification, and distribution patterns observed using antibodies from alternate vendors.
Immunofluorescence
This antibody works very well by immunofluorescence in human cells at 1:200 to 1:400 dilution. Samples were first fixed and extracted for 10 minutes at room temperature in 4% formaldehyde and 0.1% Triton X100 in 1 x PBS before briefly blocking with 3% BSA in 1 x PBS-Tween for 30 minutes followed by 1 hour with the primary antibody (diluted in 1 x PBS-Tween, 1% BSA) and 1 hour with the secondary Alexa-488 conjugated secondary goat anti-rabbit (diluted in 1 x PBS-Tween, 1% BSA). Samples were post-fixed before application of anti-fade containing DAPI.
Review Date: 31 Jan 2017 | Active Motif
Very reproducible and reliable antibody
Immunofluorescence
This is a great antibody for detecting H3K4me2 in the nucleus of human tissue culture cells or on cytospun metaphase chromosomes. The background is low and the signal strength is high. We typically use this at a 1:200 dilution.
Review Date: 16 Sept 2016 | Active Motif