Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Methylation of histone H3 at lysine 4 is linked to transcriptional activation through the recruitment of a number of downstream effector proteins (CHD1, Isw1, ING2, WDR5). At least one protein that selectively binds histone H3 trimethylated at lysine 4 has also been reported (NURF301). Trimethyl lysine 4 is enriched at the transcriptional start site of activated genes. Lysine 4 methylation is dependant upon the monoubiquitylation of the C-terminus of histone H2A.
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Not Species Specific, Human, Mouse, Yeast
- Isotype: Serum
- Quantity: 100 ul
- Storage: Antibodies in solution can be stored at -20C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Chromatin Immunoprecipitation (ChIP)
- Dot Blot (DB)
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
- Western Blotting (WB)