Histone H3K4me3 rabbit antibody (pAb)
Product Details
- Cat. No.
- 39915
- Type
- Primary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
The supplier does not provide quotations for this antibody through SelectScience. You can search for similar antibodies in our Antibody Directory.
Description
Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Methylation of histone H3 at lysine 4 is linked to transcriptional activation through the recruitment of a number of downstream effector proteins (CHD1, Isw1, ING2, WDR5). At least one protein that selectively binds histone H3 trimethylated at lysine 4 has also been reported (NURF301). Trimethyl lysine 4 is enriched at the transcriptional start site of activated genes. Lysine 4 methylation is dependant upon the monoubiquitylation of the C-terminus of histone H2A.
Biological Information
- Clonality: Polyclonal
- Host: Rabbit
- Reactivity: Not Species Specific, Human, Yeast
- Isotype: IgG
Handling
- Quantity: 100 ug
- Storage: Antibodies in solution can be stored at -20C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Specificity: 5
Applications
- Chromatin Immunoprecipitation (ChIP)
- Dot Blot (DB)
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
- Western Blotting (WB)
I tried only one lot that perfectly works as I was told be the R & D that it was validated and it stands.
ChIP and IF
Its an amazing antibody and highly specific. The background is very minimal. I tried indifferent cell types in my project which still gave the same results. I generally go by the validated antibody for ChIPseq purpose, this was already validated by active motif which I can see the results in my hand too.
Review Date: 30 Jan 2017 | Active Motif
Very sensitive, low background.
ChIP-seq, ChIP
Used in ChIP and ChIP-seq at 1ug/0.5M cells. Very sensitive while still keeping the background very low. Very reproducible as well. The PCR enrichments of positive regions over negative are often above thousand fold. We have been using it for over a year regularly and never had any problems - it is now used in our lab as the positive control during method development.
Review Date: 16 Sept 2016 | Active Motif