Product & ReviewsAntibodies
Hsp70 / Hsc70 Antibody (13D3)
Product Details
- Cat. No.
- NB120-2788
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b03d5b5719efea7eeb22049bf24d55276a9361ee-400x203.jpg?w=1080&q=75&fit=clip&auto=format)
Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b03d5b5719efea7eeb22049bf24d55276a9361ee-400x203.jpg?w=256&q=75&fit=clip&auto=format)
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Description
Hsp70 / Hsc70 Antibody (13D3)
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Bovine, Cat (Feline), Human, Mouse
- Antigen: Hsp70 / Hsc70
- Isotype: IgM
- Source: Ascites
Handling
- Quantity: 0.05 ml
- Storage: Store at -20C. Avoid freeze-thaw cycles.
- Buffer: PBS
Applications
- Blocking (BA)
- Flow Cytometry (FC)
- Immunocytochemistry (ICC)
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Immunoprecipitation (IP)
- Western Blotting (WB)
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b03d5b5719efea7eeb22049bf24d55276a9361ee-400x203.jpg?w=3840&q=75&fit=clip&auto=format)