HUMAN ANTI AKT1

Human anti Human AKT1 antibody, clone AbD02147 recognizes AKT1, a cytoplasmic kinase which is expressed in the nucleus after activation by integrin-linked protein kinase 1 (ILK1). Clone AbD02147 does not bind to human AKT2 and AKT3 (81% and 82% identical to AKT1, respectively).Two serine/threonine kinases, designated AKT1 and AKT2 exhibit sequence homology with the protein kinase A and C families and are encoded by the c-AKT proto-oncogene. AKT1 and the related AKT2 are activated by platelet-derived growth factor (PDGF). The activation is rapid and specific, and it is abrogated by mutations in the Pleckstrin homology domain (PH) of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.Additionally, the activation of AKT1 and AKT2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 is dephosphorylated by PH domain leucine-rich repeat protein phosphatase (Gao et al. 2005).

• Clonality: Monoclonal
• Host: Human
• Reactivity: Human, Mouse, Rat
• Isotype: HuCAL Fab bivalent

• Quantity: 0.1 mg
• Storage: +4ºC, -20ºC if preferred
• Buffer: Phosphate buffered saline, 0.01% Thiomersal

• ELISA (ELISA)
• Western Blotting (WB)