Immunofluorescent analysis of ( -20? Ethanol ) fixed HepG2 cells using 10298-1-AP(Lamin A/C antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Lamin A/C is also named as LMNA, FPL, LFP, EMD2, FPLD, HGPS, LDP1, LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation(PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.
Excellent antibody! Will strongly recommend.
Western blot
Antibody was tested for western blot application in a dilution 1:1000. Very specific, can be used in higher dilutions.
Review Date: 30 Aug 2018 | Proteintech Group
