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ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1.Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting.
Awesome results
Western blot
It is easy to handle, very sensitive, holds at 4c to working stock, and nicely represents the bands.
Review Date: 13 Jan 2024 | Merck KGaA, Darmstadt, Germany
I have tried many anti Actin antibodies, so far this one is the best, it is super sensitive, highly concentrated and consistently high quality and reliable.
Western blot
The best affordable actin antibody for loading control.
Review Date: 20 Feb 2021 | Merck KGaA, Darmstadt, Germany
