MOUSE ANTI DOG CD4

Mouse anti Dog CD4 antibody (CA13.1E4), a monoclonal antibody specific for canine CD4. CA13.1E4 was clustered at the first Canine Leukocyte Antigen Workshop (Claw) [Cobbold et al. 1992 ] and identifies, by immunoprecipitation a ~60 kDa monomeric protein under both reducing and non-reducing conditions. Canine CD4 is a surface glycoprotein expressed by non CD8 expressing T lymphocytes, with similar developmental and functional characteristics to T helper cells described in other mammalian species. Mouse anti Dog CD4 also recognizes a population of CD8 positive cells in the canine thymus (Moore et al. 1992). CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8 (Bismarck et al. 2012 ). Similar reports have been made for cynomolgus monkey (Nam et al. 2000), pig (Saalmuller et al. 1987 , Pescowitz et al. 1990) as well as rat, chicken and human (reviewed Zuckermann 1999 ).Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species (Moore et al. 1992). Clone CA13.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Dog (Canine)
• Isotype: IgG1

• Quantity: 2 ml
• Storage: +4ºC, -20ºC if preferred
• Buffer: 0.1% Sodium Azide

• Flow Cytometry (FC)
• Immunohistochemistry (Frozen Sections) (IHC (Fr))
• Immunoprecipitation (IP)