MOUSE ANTI HUMAN CYTOCHROME C

Mouse anti human Cytochrome C antibody, clone 2G8 recognises cytochrome c, originally termed Apaf-2, an evolutionarily conserved protein which acts as an essential mitochondrial electron carrier between respirable substrates and oxygen, during aerobic energy production.A second major function for cytochrome c is its role as an intermediate in apoptosis, both through its association with inositol-3-phosphate receptor (IP3 receptor) which acts as an ER calcium release channel, and its association with apoptotic protease activating factor 1 (Apaf-1), resulting in the formation of the apoptosome protein complex which then recruits and activates pro-caspase 9 (Apaf-3).Mouse anti human Cytochrome C antibody, clone 2G8 is reported to block the role of cytochrome c as a co-factor in the activation of caspase 9 (Neame et al. 1998) . Removal of sodium azide is recommended prior to use in functional assays – Bio-Rad recommend the use of EQU003 for this purpose.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human, Mouse, Rabbit (Lapine), Rat
• Isotype: IgG1

• Quantity: 0.1 mg
• Storage: +4ºC, -20ºC if preferred
• Buffer: Phosphate buffered saline, 0.09% Sodium Azide

• ELISA (ELISA)
• Flow Cytometry (FC)