MOUSE ANTI HUMAN p53 (aa181-190)
Product Details
- Cat. No.
- MCA1704
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
Mouse anti Human p53 antibody, clone DO-11 recognizes the human Cellular tumor antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637).DO-11 binds to an epitope within the central region of p53 between amino acids 181 - 190 defining a cryptic epitope exposed in unfolded/ denatured p53 (Shimitzu et al. 2006). Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. Mouse anti Human p53 antibody, clone DO-11 recognizes mutant forms of p53 (Warnock et al. 2011) and has been used successfully for detection of p53 by western blotting (Wei et al. 2012).
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Human
- Isotype: IgG1
Handling
- Quantity: 0.1 mg
- Storage: +4ºC, -20ºC if preferred
- Buffer: Phosphate buffered saline, 0.09% Sodium Azide
Applications
- Immunohistochemistry (Frozen Sections) (IHC (Fr))
- Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
- Immunoprecipitation (IP)
- Western Blotting (WB)