MOUSE ANTI NFkB p65

Mouse anti NFkB p65 antibody, clone 2A12A7 recognizes Nuclear factor NF-kappa-B p65 subunit also known as Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 or RelA. NF-kappa-B p105 and p100 subunits are large precursor proteins that are cleaved by the ubiquitin/proteasome pathway into the mature NF-kappa-B p50 and p52 subunits (NF-kappa-B p105 cleavage results in the p50 unit, while cleavage of NF-kappa-B p100 results in the p52 subunit). Rel protein family members interact with p52 and p50 to form transcription factors. In the absence of stimulation an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1

• Quantity: 50 µg
• Storage: -20ºC only
• Buffer: Phosphate buffered saline, 0.09% Sodium Azide, 0.01% BSA, 50% Glycerol
• Specificity: 5

• Immunohistochemistry (Paraffin-Embedded Sections) (IHC (P))
• Immunoprecipitation (IP)
• Western Blotting (WB)