Western blot analysis of Jurkat cell lysate probed with Mouse anti NFkB (p65) antibody.
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Mouse anti NFkB p65 antibody, clone 2A12A7 recognizes Nuclear factor NF-kappa-B p65 subunit also known as Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 or RelA. NF-kappa-B p105 and p100 subunits are large precursor proteins that are cleaved by the ubiquitin/proteasome pathway into the mature NF-kappa-B p50 and p52 subunits (NF-kappa-B p105 cleavage results in the p50 unit, while cleavage of NF-kappa-B p100 results in the p52 subunit). Rel protein family members interact with p52 and p50 to form transcription factors. In the absence of stimulation an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
perform well at low concentrations
WB, immunohistochemestry
A sensitive antibody with high quality and specificity.
Review Date: 26 May 2023 | Bio-Rad
