MOUSE ANTI PHOSPHOTYROSINE
Product Details
- Cat. No.
- MCA2472
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Description
Mouse anti Phosphotyrosine antibody, clone PY20 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.
Biological Information
- Clonality: Monoclonal
- Host: Mouse
- Reactivity: Chemical
- Isotype: IgG2b
Handling
- Quantity: 0.5 mg
- Storage: +4ºC, -20ºC if preferred
- Buffer: Borate buffered saline, 0.09% Sodium Azide
Applications
- ELISA (ELISA)
- Immunofluorescence (IF)
- Western Blotting (WB)