MOUSE ANTI PIG CD335:Alexa Fluor® 488

Mouse anti Pig CD335 antibody, clone VIV-KM1 recognizes the porcine homologue of human CD335, also known as NKp46 and natural cytotoxicity triggering receptor 1 (NCR1), a member of the natural cytotoxicity receptor (NCR) family.CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor and is involved in the control of viral infection and tumor development.Until recently little has been known about porcine and veterinary NK cells. CD335 is expressed by human natural killer cells (Sivori, S. et al.1997) and the development of monoclonal antibodies to bovine CD335 (clone ASK1) (Storset et al. 2004) and ovine CD335 (clone EC1.1) (Connelley et al.2011) have enabled researchers to identify and better understand ruminant NK cells. Clone VIV-KM1 is the first monoclonal developed to specifically identify porcine CD335 and provides a reagent to facilitate a better understanding of the pig immune system and aid in the understanding of the role of NK cells in host pathogen defense. Studies using VIV-KM1 have shown that, within the pig, CD335 is not universally expressed by all NK cells and that expression of this marker on NK cells may be influenced by cytokine production (Mair et al. 2012).In addition to clone VIV-KM1, clones AKS1, which recognizes CD335 (NKp46) in bovine and other ruminants, and EC1.1, which recognizes ovine and caprine CD335, are also available from Bio-Rad.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Pig (Swine)
• Isotype: IgG1

• Quantity: 100 TESTS/1ml
• Storage: +4ºC, -20ºC if preferred
• Buffer: Phosphate buffered saline, 0.09% Sodium Azide, 1% BSA

• Flow Cytometry (FC)