Product & ReviewsAntibodies

MOUSE ANTI PIG GRANULOCYTES

Product Details

Cat. No.
MCA2599GA
Type
Primary Antibody
Clonality
Monoclonal
Host
Mouse
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Bio-Rad

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Description

Mouse anti Pig Granulocytes antibody, clone 6D10 recognizes a ~60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2 (MCA2600), allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+ mature neutrophils and 6D10-2B2+ mature eosinophils and basophils.Mouse anti Pig Granulocytes antibody, clone 6D10 has been shown as suitable for use on cytospins (Perez et al. 2007).

Biological Information

  • Clonality: Monoclonal
  • Host: Mouse
  • Reactivity: Pig (Swine)
  • Isotype: IgG2a

Handling

  • Quantity: 0.1 mg
  • Storage: +4ºC, -20ºC if preferred
  • Buffer: Phosphate buffered saline, 0.09% Sodium Azide

Applications

  • Flow Cytometry (FC)
  • Immunoprecipitation (IP)
  • Western Blotting (WB)
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