MOUSE ANTI SHEEP CD25:FITC

Mouse anti sheep CD25, clone 9.14 recognizes the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Rα), a single pass type I membrane protein expressed by activated ovine T-cells.Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132). A non-covalent association of the α and β subunits is required to form the high affinity receptor for IL-2.Immunoprecipitation studies have demonstrated that clone 9.14 precipitates a band of approximately 47 kDa under reducing conditions, which is that expected of the mature protein due to high glycosylation, and is consistent with the observed molecular weight of IL-2Rα in other species such as humans (Verhagen et. al.1993).Research has demonstrated that antibodies to CD4, FoxP3 and CD25 may be used in studies of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi et. al. 2011). Mouse anti sheep CD25, clone 9.14 is one of a wide range of monoclonal antibodies available from Bio-Rad for ovine research and provides an important tool for the identification of ovine CD25 and may be of used in facilitating further studies of T-regs in this species.

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Sheep (Ovine)
• Isotype: IgG1

• Quantity: 0.1 mg
• Storage: +4ºC, -20ºC if preferred
• Buffer: Phosphate buffered saline, 0.09% Sodium Azide, 1% BSA

• Flow Cytometry (FC)