MOUSE ANTI SHEEP INTERLEUKIN-8

Mouse anti Ovine Interleukin-8 antibody, clone 8M6 recognizes ovine interleukin-8 (IL-8), also known as C-X-C motif chemokine 8. IL-8 is a 79 amino acid ~9-11 kDa chemoattractant for neutrophils, basophils and T-cells. IL-8 is produced by several cell types including neutrophils, monocytes and macrophages in response to inflammatory stimulation. Mouse anti ovine Interleukin-8 antibody, clone 8M6 shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha.Responses to infectious stimuli may vary among ovine species, the response to Mannheimia haemolytica, a causative agent of pneumonia, peritonitis and gangrenous mastitis in ovids, is exaggerated in Bighorn sheep (Ovis canadensis) compared to domestic sheep (Ovis aries) with significantly elevated IL-8 levels in response to infection (Herndon et al. 2010). Mouse anti ovine interleukin-8 antibody, clone 8M6 has been utilized to identify cells and cell types expressing IL-8 in inflamed porcine tissue (Laursen et al. 2014) showing here also that neutrophils are the predominant cell type expressing IL-8 whilst epithelial and endothelial cells in the vicinity of inflammatory lesions also express the cytokine. Clone 8M6 neutralizes the bioactivity of ovine IL-8. Mouse anti Ovine Interleukin-8 antibody, clone 8M6 has been used in conjunction with Rabbit anti Sheep Interleukin-8 antibody (AHP425) for the development of a sensitive ELISA to measure IL-8 concentrations in bovine samples (Cronin et al. 2015).

• Clonality: Monoclonal
• Host: Mouse
• Reactivity: Bovine, Cat (Feline), Dog (Canine), Ferret, Sheep (Ovine)
• Isotype: IgG2a

• Quantity: 0.25 mg
• Storage: +4ºC, -20ºC if preferred
• Buffer: Phosphate buffered saline, 0.09% Sodium Azide

• ELISA (ELISA)
• Flow Cytometry (FC)
• Functional Studies (Func)
• Western Blotting (WB)