PE anti-mouse Ly-6G
Product Details
- Cat. No.
- 127607
- Type
- Flow Cytometry Reagents
- Clonality
- Monoclonal
- Host
- Rat
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Description
PE anti-mouse Ly-6G; Clone: 1A8; Reactivity: Mouse; Apps: FC; Size: 50 μg
Biological Information
- Clonality: Monoclonal
- Host: Rat
- Reactivity: Mouse
- Clone: 1A8
- Conjugate: PE
- Isotype: Rat IgG2a, κ
- Gene: 546644
Handling
- Quantity: 50 microg
- Storage: 2°C-8°C
- Specificity: 4
Applications
- Flow Cytometry (FC)
Consistently performs well with easy identification, low bleaching and accurate/specific labelling
flow cytometry, in vivo cell labelling and identification
We use this antibody for two different techniques. Firstly, ex vivo labelling for flow cytometry. For this purpose, the Ly6G-PE antibody gives a good population of positive cells that can be clearly separated from the negative population. It is specific to the point that it allows detection of both the Ly6G-high and Ly6G-intemermediate cell populations. It is lowly expressed by some eosinophils which is worth noting, and for flow purposes for neutrophil analysis Siglec-F positive eosinophils needs to be gated out for analysis. This antibody goes well in a flow panel and identifies the neutrophil populations with a high specificity. For in vivo labelling and identification by intravital microscopy, this antibody works very well. It rapidly labels the circulating neutrophils when administered intravenously with minimal background and non-specific binding. It also copes well when cells are removed, fixed, and analysed by flow cytometry or imaging that is non-intravital.
Review Date: 22 Jun 2016 | BioLegend
